Kinetic transcriptomic approach revealed metabolic ...
2012-10-1 · Frozen samples were ground into powder in a ball-crusher. RNA extractions were performed with the RNeasy ® Plant Mini Kit (QIAGEN). RNA were finally eluted in 50 μL of RNase free water and precipitated with two volumes of absolute ethanol and 0.1 volume of 3 M sodium acetate (pH 5).